Definition of serial dilution

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A ten-fold dilution decreases an amount in a suspension or viral suspension by one ratio of ten, which is equal to one-tenth of the concentration at which it was originally found. A sequence of ten-fold Dilutions is known as ten-fold serial dilutes. In this manual, ten-fold serial dilutes are employed in titrations of the suspension of Newcastle disease virus to determine the amount of infection.

The tests are conducted in tiny sterile test tubes. The tubes are generally constructed of glass, and it is recommended that they have lids that are fitted to limit the risk for contamination in the dissolution. A twofold dilution decreases the concentration of the solution by a factor of two, which decreases the original concentration by one-half.

Two-fold Dilutions is known as two-fold serial dilutes. In this manual, twofold serial dilutions take place in tiny volumes on microwell plates. They are employed in both haemagglutination and haemagglutination inhibition tests in order to determine the levels of test samples. For a tenfold dilution to be complete the ratio should be The one represents the quantity of sample that is added.

The 10 is the amount of the finished sample. For instance an amount of 1 milliliter is then added to 9 ml of diluent , to make 10 milliliters. Decimal numbers may be transformed into scientific notations by shifting the decimal number by the same amount as the exponential number. Multiple dilutions are necessary to reduce the concentration of the sample in multiple ways.

The reduction in concentration by using less dilutions is achievable by using large-volume diluting. This is done by using the dilution instead How satisfied are you with this article?

Name Email. Sourav Bio. This article writter by Sourav Bio on April 22, Find out more article Last updated on December 8th, ,. Report Feedback Login Please. Steps of A ten-fold dilution. Steps of Ten-fold serial dilutions. Steps of A two-fold dilution. Steps of Two-fold serial dilutions. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics. In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample.

As, for instance, the number and size of bacterial colonies that grow on an agar plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids for comparing concentrations of two organisms or cell types in the sample or wells of a given volume for absolute concentrations , dilution can be useful for getting more manageable results.

Serial dilution is one of the core foundational practices of homeopathy, with 'succussion', or shaking, occurring between each dilution. In homeopathy, serial dilutions called potentisation are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain.

A small amount of serum or solute can be serially diluted by transferring aliquots to diluent. A serial dilution is a kind of solution dilution. A more exact serial dilution definition is that it is a stepwise dilution of a solution, that is repeated a certain number of times and in which the concentration decreases with each step.

The dilution factor calculator at each step does not have to be constant, but it is for this calculator. In biology and medicine [ edit ] In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample.